How the methods actually work
Answer-first technique guides for cloning and structure-based drug discovery: when to use a method, the numbers that matter, and a worked example.
Gibson Assembly
Gibson Assembly protocol: 0.02–0.5 pmol of fragments, 1:2 vector to insert, 50 °C for 15–60 min.
Open the Gibson Assembly wizard
Golden Gate Assembly
Golden Gate Assembly protocol: 0.05 pmol of each part, BsaI-HFv2 with T4 ligase, 30 cycles of 37 °C and 16 °C.
Open the Golden Gate wizard
Use your own model in Talindrew, including AlphaFold 3
Plug your own model into any FoldRx stage: the request and response contract, 202 polling, tokens, and a one-file adapter for your licensed AlphaFold 3..
Open the AI workspace
Can you use AlphaFold 3 commercially?
AlphaFold 3 weights are non-commercial; the code is Apache 2.0.
Fold with Boltz-2 or your own AlphaFold 3
ESMFold2 vs AlphaFold 3 vs Boltz-2
ESMFold, ESMFold2, AlphaFold 3 and Boltz-2 compared: MSA or single sequence, ligands, speed, confidence, licences, and which to use for drug discovery..
Compare models in the AI workspace
Structure-based virtual screening, step by step
A structure-based virtual screening workflow in order: structure, pockets, library, docking, ADMET, pose checks, selectivity and ranking, with the tools..
Build this pipeline in the AI workspace
Binding pocket prediction: P2Rank vs fpocket
How ligand binding site prediction works: P2Rank's learned scoring vs fpocket's alpha spheres, choosing the pocket to dock into, and predicted structures..
Find pockets in the AI workspace
Each guide links straight into the matching wizard or the AI workspace, with a demo to step through before you touch your own construct or target.
Start with a worked example
Free to start. Runs in any modern browser, nothing to install.