How the methods actually work
Technique guides written answer-first: what the method is, when it is the right choice, the numbers that matter, and a worked example you can run in Talindrew. Every figure carries its source.
Gibson Assembly
Gibson Assembly joins DNA fragments through 15–40 bp homologous overlaps using exonuclease, polymerase and ligase in one 50 °C reaction.
Open the Gibson Assembly wizard
Golden Gate Assembly
Golden Gate Assembly uses Type IIS enzymes like BsaI to cut outside their recognition site, producing custom 4 nt overhangs for scarless, one-pot, multi-fragment cloning.
Open the Golden Gate wizard
Each guide links straight into the matching wizard, which ships a Load demo button, so you can step through the reaction on a real construct before touching your own.
Start with a worked example.
Free to start. Runs in any modern browser, nothing to install.