Every tool, already open
Primer design, multiple sequence alignment, BLAST, restriction digest, oligo calculator and gel simulator: available in one click, in context with your sequence. No switching between five different websites.
Included tools
01
Primer Design
Ranked primer pairs for any region.
Select a region and get primer pairs scored on nearest-neighbour Tm, GC clamp, hairpins, dimers, product size and binding sites, re-ranked as you move the constraints. Add restriction sites, tags or Gibson arms as 5′ overhangs.
02
Multiple Sequence Alignment
Align up to 200 sequences at once.
DNA, RNA or protein. A built-in progressive aligner with affine gaps and BLOSUM62, or MAFFT, MUSCLE and Clustal Omega when installed. Consensus, conservation, identity matrix and tree; export FASTA, CLUSTAL or Newick.
03
NCBI BLAST
Find homologues in seconds.
Submit nucleotide or protein BLAST queries directly from your open sequence. Results stream back with E-value, identity, and alignment view: no switching tabs.
04
Restriction Digest
Simulate any digest instantly.
Eleven enzyme sets drawn from REBASE with Dam, Dcm and EcoKI methylation applied. Talindrew predicts cut sites, fragment sizes, and compatible ends, then renders the result on your sequence map.
05
Oligo Calculator
Check any oligo before you order it.
Input a primer and get Tm (nearest-neighbour), GC content, hairpin and dimer detection, and a suggested annealing temperature: all in one panel.
06
Gel Simulator
Visualise your bands before you run the gel.
Configure ladder, lane count, and fragment sizes from your digest or PCR. Get a gel image you can drop straight into a lab notebook entry.
Always in context
Every tool in the Analysis Suite operates on your currently open sequence. Design primers and they are drawn on the map and saved with the construct. Run a digest and the predicted bands are highlighted. Send the open tabs to the aligner without exporting a file. There is no copy-paste, no URL bar, no context switch, just your sequence and the answer.
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