Golden Gate Assembly on a Cloning Canvas: MoClo Yeast Toolkit
Video · 1:07 · Published Invalid Date · Talindrew team
About this video
The cloning workspace, start to finish: a kit template loads its eight MoClo YTK parts, each with its own map; the parts are wired into a Golden Gate reaction with BsaI; the engine cuts every part and matches each four base overhang to the next; and the 5,459 bp circular product carries every feature over. The whole strategy saves as one portable file and shares by link or into the lab notebook.
What you will learn
- How kit templates fill a Golden Gate assembly with real parts.
- Why each four base overhang decides the order of the parts.
- How a whole strategy saves as one file and shares by link.
Transcript
- 0:00
One canvas
Cloning is a chain of steps. The workspace puts the whole chain on one canvas.
- 0:06
Kit template
Start from a kit template, like the MoClo Yeast Toolkit, and load its demo.
- 0:13
Parts
Eight parts land on the canvas, each with its own map.
- 0:18
Golden Gate reaction
Wire them into a Golden Gate reaction with BsaI.
- 0:22
Overhangs
The engine cuts every part and matches each four base overhang to the next.
- 0:30
Product
Out comes the product: five thousand four hundred and fifty nine base pairs, circular, every feature carried over.
- 0:40
Operations
Sixteen operations run on the same engine: PCR, Gibson, Gateway, CRISPR and more.
- 0:48
Save and load
Every strategy saves as one portable file, with its sequences, primers and history.
- 0:55
Share and notebook
Share it by link, or save the reaction into your lab notebook. Talindrew. Plan the whole strategy on one canvas.
Try it on your own sequences.
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