The Talindrew Workbench: A Tour of the Real App

Video · 1:29 · Published Invalid Date · Talindrew team

About this video

The real app, tool by tool, in one browser tab: open pUC19 and its map draws with every feature and cut site; read the sequence with its translation; design primer pairs ranked by melting temperature and product size; simulate the gel; scan for CRISPR guide RNAs scored beside their PAM; load a Sanger trace; align many sequences with a consensus and conservation track; plan the cloning strategy on one canvas; record it in a notebook entry with the live map; look at the protein's predicted structure coloured by confidence; and follow FoldRx, in beta, from a fold to ranked drug candidates.

Structures: AlphaFold DB models AF-P42212-F1 and AF-P00519-F1 (EMBL-EBI), CC BY 4.0.

What you will learn

Which tools Talindrew puts in one tab, from sequence editing to protein folding.
How a plasmid, its primers, its gel and its clone verification connect.
Where the cloning canvas, the lab notebook and the Model Hub fit in.

Transcript

0:03
The workbench
This is Talindrew: a molecular biology workbench that runs in one browser tab.
0:10
Plasmid map
Open a plasmid, and the map draws itself, with every feature and every cut site.
0:17
Sequence
Switch to the sequence to read it base by base, translated as you go.
0:23
Primer design
Select a region, and Talindrew designs primer pairs, ranked by melting temperature and product size.
0:30
Gel simulation
Simulate the gel before you touch a pipette.
0:36
CRISPR guides
Scan for guide RNAs, each one scored beside its PAM.
0:41
Sanger traces
Drop in a Sanger trace to check your clone.
0:47
Alignment
Align many sequences at once, with a consensus and a conservation track.
0:53
Cloning canvas
Plan the whole cloning strategy on one canvas, from parts to product.
1:00
Lab notebook
Record it in a lab notebook, with the live map inside the entry.
1:06
Protein structure
Then look at the protein you built, coloured by confidence.
1:12
FoldRx pipeline
And in beta, FoldRx runs a drug discovery pipeline, from the fold to ranked candidates. Talindrew. Design, clone and fold, in one tab. Free to start.